Microbiome Analysis

Labwork:

Overview Microbiome Analysis Experiment
Meaning of human microbiomes e. g. in the colon and on the skin
Introduction about PCR to amplify 16S r-RNA-gene
Introduction agarose gel electrophoresis with Lonza ready gels and electrophoresis tools
Purification of PCR products by SPRI
Microbiome Preperation
PCR and Libary preparation
Preparation for Flongle Flow Cell
Flongle Flow Cell Priming
Start Sequencing and enter set-up
Basecalling Epi2me
Basecalling Epi2me
Results Presentations for microbiome analysis