Microbiome Analysis

Labwork:

Overview Microbiome Analysis Experiment
Structure of DNA
Meaning of human microbiomes e. g. in the colon and on the skin
Introduction about PCR to amplify 16S r-RNA-gene
Purification of PCR products by SPRI
Introduction agarose gel electrophoresis with Lonza ready gels and electrophoresis tools
Preparation for Microbiome Analysis
Microbiome Analysis
Preparation for Flongle Flow Cell
Flongle Flow Cell Priming
Start Sequencing and enter set-up
Basecalling Epi2me
Results Presentations for microbiome analysis